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Layered rainbow Jell-O mold on a serving plate, sliced to show colorful layers.

Aunt Penny’s Harvest Jell-O

A layered rainbow Jell-O with sour cream - fun, festive, and perfect for Thanksgiving. A nostalgic dessert that delights kids and adults alike.
Bea Citron  |  September 15, 2025

Aunt Penny’s Harvest Jell-O

A colorful, layered Jell-O dessert with sour cream—guests of all ages will love this festive, cool addition to the Thanksgiving table.

Layered rainbow Jell-O mold on a serving plate, sliced to show colorful layers.

Ingredients

  • 12 small (regular size) boxes of Jell-O (flavors for alternating colors)
  • 4 strawberry or strawberry-banana
  • 4 orange
  • 4 lime
  • 4 lemon
  • 9” round cake pan with straight sides (at least 3” deep)
  • 2 large containers of sour cream
  • Canola oil (for greasing pan)

Directions

  1. Lightly grease the bottom and sides of the cake pan with canola oil.
  2. Dissolve the first box of Jell-O in 1 cup boiling water. Pour 2/3 cup of this mixture into the pan. Reserve the remaining 1/3 cup. Refrigerate for 30 minutes until set, ensuring the pan sits flat.
  3. Combine the reserved 1/3 cup Jell-O with 1/3 cup sour cream. Whisk until smooth. If mixture thickens too much, gently rewarm. Pour this over the first set layer. Refrigerate until firm.
  4. For each subsequent box of Jell-O, repeat the process—pouring 2/3 cup plain Jell-O layer, then 1/3 cup mixed with sour cream, alternating colors (red, orange, green, yellow, etc.). Allow each layer to chill at least 30 minutes before adding the next.
  5. Continue layering until the pan is filled.
  6. Before serving, run a sharp knife around the pan’s edge. Place the pan briefly in 1” of hot tap water and gently shimmy until loosened. Invert onto a serving dish to release.
  7. Slice and serve to reveal the rainbow layers inside!

Notes

Use a 9” round cake pan with straight sides, at least 3 inches deep. This recipe takes 2–3 days to prepare since each layer must fully set before adding the next.

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